Inhibition of let-7c Regulates Cardiac Regeneration after Cryoinjury in Adult Zebrafish

Inhibition of let-7c Regulates Cardiac Regeneration after Cryoinjury in Adult Zebrafish

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The let-7c family of micro-RNAs (miRNAs) is expressed during embryonic development and plays an important role in cell differentiation.We have investigated the role of let-7c in heart regeneration after injury in adult zebrafish.let-7c antagomir or scramble injections were given at one day after cryoinjury (1 dpi).

Tissue samples were collected at 7 dpi, 14 dpi and 28 dpi carolina force softball and cardiac function was assessed before cryoinjury, 1 dpi, 7 dpi, 14 dpi and 28 dpi.Inhibition of let-7c increased the rate of fibrinolysis, increased the number of proliferating cell nuclear antigen (PCNA) positive cardiomyocytes at 7 dpi solar filter 55mm and increased the expression of the epicardial marker raldh2 at 7 dpi.Additionally, cardiac function measured with echocardiography recovered slightly more rapidly after inhibition of let-7c.

These results reveal a beneficial role of let-7c inhibition in adult zebrafish heart regeneration.